葛根素对双酚S致睾酮合成损伤的保护作用

阚 雅琪, 吴 梓瑶, 覃 美琳, 周 靖刚, 潘 梦芬, 曾怀 才*
桂林医学院公共卫生学院广西环境暴露组学与全生命周期健康重点实验室

摘要


目的：研究双酚 S（BPS）对睾丸间质细胞睾酮合成的抑制作用与机制及葛根素（PUE）的干预作用。
方法：用 25、50、100 mg/L BPS处理小鼠睾丸间质细胞（TM3）48 h并用 30 mg/L PUE进行干预，ELISA法检测睾
酮水平，试剂盒检测检测氧化应激指标 CAT、GSH、SOD、MDA和ROS的水平，Western blot检测细胞中 StAR和
MAPK信号通路相关蛋白表达水平。结果：BPS处理 48 h后，TM3细胞活性降低且呈剂量依赖性，同时睾酮水平降
低，经 PUE干预后睾酮水平增加。BPS引起细胞中 SOD、CAT和 GSH的活力降低，而ROS和MDA水平增加，而
PUE干预后抗氧化剂的活力增加，ROS和MDA水平降低。BPS暴露引起 StAR蛋白表达水平降低（P＜ 0.05），经过
PUE处理后 StAR水平增加。BPS处理后 p38/JNK MAPK信号通路上相关蛋白表达水平升高，磷酸化水平升高，PUE
干预后MAPK信号通路磷酸化水平降低。结论：BPS诱导TM3细胞发生氧化应激，激活 p38/JNK MAPK信号通路，
抑制睾酮合成，是BPS诱导雄性毒性的一种可能机制。PUE能减轻BPS引起的TM3细胞毒性作用。

关键词


双酚 S；睾酮；p38/JNK MAPK信号通路；氧化应激

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