探索脂质代谢基因表达与AML预后的关系

潘 慧怡, 张 静, 金润 铭*
华中科技大学同济医学院附属协和医院

摘要


目的：通过生物信息学方法探讨脂质代谢在急性髓系白血病（AML）发生发展中的作用，并对AML患者建
立危险分层方法，确定预后相关基因，从而为个体化靶向治疗提供可能。方法：从UCSC数据库下载AML患者的基
因表达RNAseq和临床资料并进行筛选。从MsigDB下载获得脂质代谢相关基因（LMRGs）。使用 ConensusClusterPlus
聚类法确定新的分子亚群。基因本体功能注释（GO）、京都基因与基因组百科全书（KEGG）、基因集变异分析
（GSVA）和Metascape数据库进行功能分析。Estimate算法和 CIBERSORTx确定骨髓微环境中的免疫状态和免疫细胞
相对丰度。Lasso回归和多因素 Cox回归分析建立预后风险模型。结果：本研究基于 LMRGs表达和临床数据鉴定出两
个存活率有显著差异的亚组。较好的预后与间质评分低、免疫评分低、免疫状态相对较低有关。Metascape富集分析
显示，两个亚组之间的差异表达基因（DEG）主要集中在免疫和骨重建相关的通路上。GSVA分析表明，脂质分解
代谢和脂质羟基化可能影响免疫和骨重建。基于 LMRGs构建的预后风险模型在预测AML患者生存显示出一定的准
确性。结论：LMRGs的表达与AML患者的免疫和骨重建和有关，可用于对AML患者危险分层以及预测生存。

关键词


AML；脂代谢；骨髓微环境；骨重建；预后

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