压力影响硬脑膜细胞迁移和增殖

陈 奇1, 王 羽1, 王 润泽1, 冯 子龙1, 赵 志军1, 李 文斌2
1、内蒙古科技大学包头医学院;内蒙古科技大学包头医学院第一附属医院神经外科;包头医学院神经外科疾病研究所（转化医学）;内蒙古自治区骨组织再生与损伤修复工程技术中心
2、包头市国药北方医院神经外科

摘要


目的：通过建立硬脑膜细胞压力加载模型，观察硬脑膜细胞在压力条件下的增殖和迁移能力的变化，为
揭示颅内压在硬脑膜发育中的作用提供新方向。方法：（1）CCK-8观察硬脑膜细胞加压/未加压培养后的增殖能
力变化；（2）细胞划痕实验和Transwell细胞迁移实验观察未成熟硬脑膜细胞加压/未加压培养后迁移能力的变化。
结果：（1）培养的未成熟硬脑膜符合鉴定标准并能稳定传代；（2）CCK-8结果显示在早期（8h内）压力促进硬脑膜
增殖，8h后压力抑制硬脑膜细胞增殖（P＜0.05）；（3）细胞划痕实验结果显示，与未加压培养组（Control组）相比
压力培养组硬脑膜细胞迁移受到抑制（P＜0.05），Transwell细胞迁移实验与细胞划痕结果相同。结论：（1）成功建
立硬脑膜细胞压力加载模型；（2）验证硬脑膜增殖和迁移能力受到压力调节。

关键词


未成熟硬脑膜细胞；压力；细胞增殖；细胞迁移；颅内压

全文:

PDF


参考


[1]AL-JEZANI N, CHO R, MASSON A O, et

al. Isolation and Characterization of an Adult Stem Cell

Population from Human Epidural Fat [J]. Stem cells

international, 2019, 2019: 2175273.

[2]SHAN J, XIAOQIAN D, XIA L, et al. Oxidative

stress, autophagy, and apoptosis induced by doxycycline

in loach fin cells in vitro [J]. The Science of the total

environment, 2022, 839: 156379.

[3]ZHU S, ZHANG Q, XU X, et al. Recent Advances

in Silk Fibroin-Based Composites for Bone Repair

Applications: A Review [J]. Polymers, 2025, 17(6).

[4]BI X, MAO Z, LI L, et al. Janus decellularized

membrane with anisotropic cell guidance and anti-adhesion

silk-based coatings for spinal dural repair [J]. Nature

communications, 2025, 16(1): 1674.

[5]ZOERLE T, BEQIRI E, ÅKERLUND C A I, et

al. Intracranial pressure monitoring in adult patients with

traumatic brain injury: challenges and innovations [J]. The

Lancet Neurology, 2024, 23(9): 938-50.

[6]ZHAO F, ZHU J, DONG X, et al. The Influence of

Extracellular Vesicles Secreted by Dural Cells on Osteoblasts

[J]. Molecular biotechnology, 2024, 66(12): 3674-87.

[7]TANG Y, ZHAO C, ZHUANG Y, et al.

Mechanosensitive Piezo1 protein as a novel regulator in

macrophages and macrophage-mediated inflammatory

diseases [J]. Frontiers in immunology, 2023, 14: 1149336.

[8]SANG W, URAL A. Influence of Osteocyte

Lacunar-Canalicular Morphology and Network Architecture

on Osteocyte Mechanosensitivity [J]. Current osteoporosis

reports, 2023, 21(4): 401-13.

[9]SWIATLOWSKA P, TIPPING W, MARHUENDA

E, et al. Hypertensive Pressure Mechanosensing Alone

Triggers Lipid Droplet Accumulation and Transdifferentiation

of Vascular Smooth Muscle Cells to Foam Cells [J]. Advanced

science (Weinheim, Baden-Wurttemberg, Germany), 2024,

11(9): e2308686.

[ 1 0 ] S A R A S W A T H I B H A T L A A , I N D A N A

D, CHAUDHURI O. Cell-extracellular matrix

mechanotransduction in 3D [J]. Nature reviews Molecular cell

biology, 2023, 24(7): 495-516.


Refbacks

  • 当前没有refback。