细胞系交叉污染鉴别的技术范式与科学逻辑 ——从形态学到基因组学的系统性整合研究

郭 文洁, 申 飞宇, 谭 雯文, 曹 雅林, 陈吉 恒*
广西医科大学

摘要


人源细胞系细胞交叉污染现象在上世纪60年代首次被报道，直到今天仍在世界范围内广泛存在并给全球细
胞实验室带来重大挑战。人源细胞系交叉污染的普遍程度令人担忧，据估计，高达30%的细胞系可能存在错误鉴定
的情况，这不仅导致了大量的经济损失，还加剧了确保实验可重复性和数据有效性的复杂性。为了应对日益严重的
细胞交叉污染和错误鉴定问题，研发和应用可靠的细胞鉴定方法成为了行业热点。本文回顾了主要的针对人源细胞
系所使用的细胞鉴定方法，并对各鉴定方法的机制、优缺点、研究进展以及发展方向进行概述，以期为细胞研究从
业人员提供人源细胞鉴定方法选择的参考。

关键词


人源细胞系；细胞系身份鉴定；短串联重复序列

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