KLT通过PI3K/AKT/mTOR通路抑制顺铂耐药鼻咽癌CNE2/DDP细胞迁移

胡 成达, 邹 茜茜, 何 俊豪, 谭 曦, 潘桂 洪*
湖南医药学院

摘要


目的：探讨康莱特注射液（KLT）对顺铂耐药鼻咽癌CNE2/DDP细胞迁移的影响及机制。方法：采用CCK-8法、划痕实验及Western blotting分别检测细胞增殖、迁移及E-cadherin、N-cadherin与PI3K/Akt/mTOR通路相关蛋白表达。结果：康莱特注射液可浓度依赖性抑制CNE2/DDP细胞增殖（P

关键词


康莱特注射液；CNE2/DDP细胞；迁移；PI3K/Akt/mTOR通路

全文:

PDF


参考


[1]Ni C, Li B, Ding Y, et al. Anti-Cancer Properties of Coix Seed Oil against HT-29 Colon Cells through Regulation of the PI3K/AKT Signaling Pathway. Foods. 2021;10(11):2833. Published 2021 Nov 17.

[2]袁艳艳，蓝苑钊，崔毅等.枞酸在顺铂耐药鼻咽癌细胞增殖、侵袭、迁移中的作用及分子机制[J].临床耳鼻咽喉头颈外科杂志，2024，38（03）：188-194+199.

[3]Guan S, Wei J, Huang L, Wu L. Chemotherapy and chemo-resistance in nasopharyngeal carcinoma. Eur J Med Chem. 2020;207:112758.

[4]Li H, Peng L, Yin F, et al. Research on Coix seed as a food and medicinal resource, it's chemical components and their pharmacological activities: A review. J Ethnopharmacol. 2024;319(Pt 3):117309.

[5]吕品田，萧娟，周坤，王亚珍，刘斌.薏苡仁注射液对肺腺癌细胞多药耐药性逆转作用的机制研究[J].中国全科医学，2010，13（26）：2956-2958.

[6]Yang C, Hou A, Yu C, et al. Kanglaite reverses multidrug resistance of HCC by inducing apoptosis and cell cycle arrest via PI3K/AKT pathway[J]. Onco Targets Ther, 2018, 11: 983-996.

[7]Zhang X-W, Liu L, Zhang X-Z, et al. Kanglaite inhibits the expression of drug resistance genes through suppressing PVT1 in cisplatin-resistant gastric cancer cells[J]. Experimental and Therapeutic Medicine, 2017, 14(2): 1789-1794.

[8]Pistritto G, Trisciuoglio D, Ceci C, et al. Apoptosis as anticancer mechanism: function and dysfunction of its modulators and targeted therapeutic strategies[J]. Aging-Us, 2016, 8(4): 603-619.

[9]王韬，费建东，聂双发等.薏苡仁油联合5-FU对结肠癌HCT-116细胞株增殖、凋亡的影响[J].西部中医药，2024，37（01）：9-12.

[10]史亚波，周梦娜，刘华兵等.康莱特注射液对人胆管癌细胞RBE和HCCC9810生长、凋亡、迁移的影响[J].实用中西医结合临床，2023，23（11）：1-5+13.

[11]朱广辉，郑琦，高瑞珂等.康莱特注射液调控胆固醇代谢以抑制肺腺癌A549细胞的恶性生物学行为[J].中国肿瘤生物治疗杂志，2023，30（11）：973-980.

[12]Wang Y, Zhang C, Zhang S, et al. Kanglaite sensitizes colorectal cancer cells to Taxol via NF-κΒ inhibition and connexin 43 upregulation [published correction appears in Sci Rep. 2018 Apr 12;8(1):6141]. Sci Rep. 2017;7(1):1280. Published 2017 Apr 28. doi:10.1038/s41598-017-01480-2

[13]Shi G, Zheng X, Zhang S, et al. Kanglaite inhibits EMT caused by TNF-α via NF-κΒ inhibition in colorectal cancer cells. Oncotarget. 2017;9(6):6771-6779. Published 2017 Dec 22. doi:10.18632/oncotarget.23645

[14]Wang L, Li S, Luo H, Lu Q, Yu S. PCSK9 promotes the progression and metastasis of colon cancer cells through regulation of EMT and PI3K/AKT signaling in tumor cells and phenotypic polarization of macrophages. J Exp Clin Cancer Res. 2022;41(1):303. Published 2022 Oct 14.

[15]潘桂洪.康莱特注射液逆转人鼻咽癌细胞CNE2/DDP的耐药作用[D].南华大学，2022.

[16]李丽，王强.中药调控PI3K/AKT/mTOR通路逆转肿瘤化疗耐药研究进展[J].中草药，2023，54（8）：2510-2519.


Refbacks

  • 当前没有refback。