电针调控SCF/c-kit信号通路对脊髓损伤大鼠肠功能障碍的影响

郝 郁文, 赵 凯林, 郑 玲, 张 昊, 郑海 梅*
泰安市中心医院（青岛大学附属泰安市中心医院、泰山医养中心）

摘要


目的：基于SCF/c-kit信号通路探讨电针下合穴对脊髓损伤大鼠肠道传输功能及cajal间质细胞的影响及作
用机制。方法：48只SD大鼠按随机法分为假手术组，模型组，电针组及电针加阻断剂组，每组12只。除假手术组
外均建立脊髓损伤大鼠模型，电针加阻断剂组于造模成功后次日注射阻断剂c-Kit-IN-1（注射两周），电针组与
电针加阻断剂组均于造模一周后予以电针刺激。结果：结肠电生理活动检测：模型组波幅、频率均较差于假手术
组，电针组较模型组有改善，电针加阻断剂组略差于电针组；苏木素-伊红染色：与假手术组相比，损伤组结肠肌
层及粘膜层明显增厚，治疗后，电针组肌层及粘膜层改善优于电针加阻断剂组；蛋白免疫印迹：模型组SCF、c-kit
蛋白表达较假手术组下调（P<0.05），电针组较模型组显著上调（P<0.01），但电针加阻断剂组较电针组上调程度差
（P<0.05）；免疫荧光：模型组在脊髓损伤后结肠组织内Cajal间质细胞数目减少（P<0.05），治疗后，电针组较模型
组增强（P<0.05），且电针组优于电针加阻断剂组（P<0.05）。结论：电针显著改善脊髓损伤大鼠结肠组织损伤，促
进Cajal间质细胞表达，可能与调控SCF/c-kit信号通路有关。

关键词


脊髓损伤；肠功能障碍；电针；Cajal间质细胞；SCF；c-kit

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参考


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