细胞自噬通过调控上皮间质转化影响宫腔粘连的研究

胡 晨, 郭 欣
山东第一医科大学附属济南妇幼保健院

摘要


宫腔粘连（Intrauterine Adhesion，IUA）是诱发女性继发性不孕与月经异常的难治性疾病，其病理特征为子
宫内膜纤维化及上皮-间质转化（Epithelial-Mesenchymal Transition，EMT）。细胞自噬（Autophagy）在组织纤维化
进程中的关键调控作用，本研究旨在阐明自噬在IUA发生发展中的病理学意义及其对EMT的调控机制。本研究分别
构建大鼠IUA体内模型及TGF-β诱导的子宫内膜上皮细胞体外模型，并给予自噬激动剂雷帕霉素（Rapamycin）进
行干预。结果表明，IUA模型组中细胞自噬水平显著抑制，并伴随氧化应激加剧、炎症因子过度释放以及E-cadherin
表达下调，Vimentin表达上调的EMT表型改变。经雷帕霉素干预后，自噬相关标志物表达显著上调，抑制p62累积。
干预组EMT进程被有效逆转，羟脯氨酸（HYP）含量及活性氧（ROS）水平显著降低，内膜胶原沉积明显改善。综
上所述，自噬功能受损是驱动IUA内膜纤维化进展的关键因素，靶向激活细胞自噬可有效拮抗子宫内膜EMT进程，
为IUA的临床防治提供了潜在的治疗靶点与理论依据。

关键词


宫腔粘连;细胞自噬;上皮间质转化;雷帕霉素;纤维化

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参考


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