Sleeping Beauty，PiggyBac与Tol2转座子在稳定基因转染过程中的应用

吕 伊婧1, 黄 荣师2, 叶 燕焊2, 陈泰 霖*2
1、广西中医药大学基础医学院;广西中医药大学药学院
2、广西中医药大学基础医学院

摘要


DNA转座子作为高效的非病毒基因递送工具，凭借其低免疫原性、制备简单及成本低廉等优势，相比病毒
载体，其结构更为简单，易于储存和运输，在基因治疗及功能基因组学中潜力巨大。它们通过独特的“剪切-粘贴”
机制实现外源基因在宿主基因组中的稳定整合，克服了传统质粒转染瞬时表达的局限。本文综述了Sleeping Beauty
（SB）、PiggyBac（PB）和Tol2三种代表性系统的分子特性与应用进展。分析表明：SB系统作为Tc1/mariner超家族
的成员，在肿瘤基因组筛查与临床CAR-T治疗中表现高效；PB系统凭借大容量承载及特异性识别TTAA位点的
“无痕”切除特性，在细胞重编程中优势显著；Tol2系统则因无过量抑制现象、高活性及大片段装载能力，成为脊椎
动物发育生物学的重要工具。文章最后展望了转座子技术与新兴基因编辑工具的融合前景。

关键词


Sleeping Beauty；piggyBac；Tol2；稳定转染；基因治疗

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参考


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