吲哚菁绿显影小鼠胆管并益于左外叶门静脉的结扎

姚 娜1, 高 东梅1, 闫 加艳2, 周 俭*2, 罗楚 彬*2, 李 卡3
1、复旦大学附属中山医院 肝癌研究所实验室
2、复旦大学附属中山医院 肝胆肿瘤和肝移植外科
3、复旦大学附属中山医院 实验动物中心

摘要


啮齿类动物是构建动物模型时最常使用的实验工具,然而目前并没有啮齿类动物胆道系统结构的详细报道。
本文通过静脉注射吲哚菁绿溶液,并同时结扎胆总管,数分钟后可使小鼠(Mus musculus)肝外胆道系统呈翠绿色,
可见各肝叶胆管常于近胆囊处汇入胆总管。吲哚菁绿肝外胆管造影后,利用25×显微镜观察小鼠左外叶肝脏脉管系
统,将小鼠左外叶肝脏分为隐藏型、显露差型、显露一般型和显露完全型。选择显露一般型和显露完全型,用10-0
带线缝合针于左外叶脏面,包绕左外叶门静脉进针和出针,外科结结扎。手术后3天观察,左外叶肝脏鲜活,未结
扎叶肝脏Ki67阳性细胞核占比提高,肝叶重量明显增加,成功诱导了肝脏再生。

关键词


吲哚菁绿;胆管;门静脉结扎;肝再生

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参考


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