吲哚菁绿显影小鼠胆管并益于左外叶门静脉的结扎
摘要
本文通过静脉注射吲哚菁绿溶液,并同时结扎胆总管,数分钟后可使小鼠(Mus musculus)肝外胆道系统呈翠绿色,
可见各肝叶胆管常于近胆囊处汇入胆总管。吲哚菁绿肝外胆管造影后,利用25×显微镜观察小鼠左外叶肝脏脉管系
统,将小鼠左外叶肝脏分为隐藏型、显露差型、显露一般型和显露完全型。选择显露一般型和显露完全型,用10-0
带线缝合针于左外叶脏面,包绕左外叶门静脉进针和出针,外科结结扎。手术后3天观察,左外叶肝脏鲜活,未结
扎叶肝脏Ki67阳性细胞核占比提高,肝叶重量明显增加,成功诱导了肝脏再生。
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