TRPV1分子对新生鼠缺氧缺血性脑损伤的影响

管 明德, 陈 莲
苏州卫生职业技术学院 基础医学部

摘要


目的：构建和验证新生鼠缺氧缺血性脑病（HIE)模型，探究瞬时受体电位香草酸亚型I（TRPV1)在缺氧缺血性脑损伤中的影响。方法：建立HIE新生鼠模型，利用HE染色光镜观察和TUNEL染色荧光显微镜观察验证模型构建效果，进行神经元损伤程度评价。通过RT-PCR和Western Blot对HIE新生鼠模型中TRPV1的表达量进行检测。免疫组化检测TRPV1在小鼠脑区各部位的表达情况。结果：HE染色光镜观察和TUNEL染色荧光显微镜观察见HIE模型组新生鼠缺氧缺血区神经元脑损伤严重、神经元凋亡明显增多。Western Blot和RT-PCR方法检测HIE模型新生鼠缺氧缺血侧和非缺血仅缺氧侧TRPV1表达量，缺氧缺血侧TRPV1表达趋势明显增高。DAB染色观察HIE模型组同侧相关缺氧缺血敏感脑区的TRPV1表达量明显高于sham组。结论：HIE可导致明显神经组织损伤和神经元凋亡，在HIE病程中TRPV1在缺氧缺血性敏感区脑组织内表达明显上调，提示TRPV1在HIE发生发展中起重要作用。

关键词


TRPV1；新生儿缺氧缺血性脑病；神经保护

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参考


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