亚精胺抑制外泌体miRNA-451-5p的机制研究

杨 明1, 范 文娟1, 明 芳1, 程 波1, 谌 赟1, 刘 敏1, 刘 晓峰2
1、江汉大学附属医院
2、汉口医院

摘要


亚精胺（Spermidine）作为一种内源性多胺，在多种生理和病理过程中发挥重要作用，尤其在调控细胞增殖、凋亡及衰老方面具有显著功能。近年来，外泌体中miRNA在细胞间通信中的作用日益受到关注。miRNA-451-5p是一种与炎症反应、肿瘤发生及细胞代谢密切相关的小分子RNA。本研究旨在探讨亚精胺通过调控外泌体miRNA-451-5p表达的分子机制。通过采用qRT-PCR、Westernblot及纳米颗粒跟踪分析等手段，发现亚精胺处理后细胞外泌体中miRNA-451-5p表达水平显著下降，伴随炎症相关信号通路活性减弱。进一步机制研究表明，亚精胺可能通过调节miRNA加工酶Dicer或影响外泌体分泌通路实现该效应。本研究揭示了亚精胺调控外泌体miRNA的新机制，为其在炎症性疾病或癌症治疗中的应用提供了理论依据。

关键词


外泌体；miRNA-451-5p

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参考


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